Strand scission and rejoining of DNA in cultured mammalian cells induced by 4-nitroquinoline 1-oxide.
نویسندگان
چکیده
The effect of the carcinogen, 4-nitroquinoline 1-oxide, on cultured mouse fibroblasts, strain L-P3, a substrain of L-929, was studied. Cells were cultured in a proteinand lipid-free synthetic medium, DM-120. Strand breakage and repair of DNA caused by 4-nitroquinoline 1-oxide was examined. 4-Nitroquinoline 1-oxide caused singleand double-strand breaks of DNA as shown by alkaline and neutral sucrose gradient centrifugation, respectively. Breakage increased with increase in the concentration of carcinogen. At a concentration of 1 X lo"5 M 4-nitroquinoline 1-oxide, the
منابع مشابه
Scissions of proteins linking DNA in cultured mammalian cells induced by 4-nitroquinoline 1-oxide and their repair.
of breakage increased, recovery was incomplete, and the fraction which was still unrepaired after recovery incubation increased. Concomitant with repair of DNA the growth capacity of the cells was regained. However, as the unrepaired fraction increased with increase in the concentration of 4NQO, the viability of the cells decreased. Cell killing induced by 4NQO was suggested to be due to disord...
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The effects of a number of 4-nitroquinoline 1-oxide and 4-nitropyridine I-oxide derivatives, with varying carcino genic potencies, on the scission of proteins linking DNA were studied in cultured mouse fibroblasts, strain L . P3. With twenty-two 4-nitroquinoline I-oxide derivatives and twelve 4-nitropyridine 1-oxide derivatives tested, an excel lent correlation was found between the scission ef...
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عنوان ژورنال:
- Cancer research
دوره 32 6 شماره
صفحات -
تاریخ انتشار 1972